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Lab 5 Document and Submission

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Q If something went wrong with this experiment, please reach out to the instructor. 1. Many things can affect the evaporation of solvents during a chromatography experiment. To allow researchers to compare results across experiments under different conditions, scientists use an Rf value, that is the ratio of the distance the pigment travelled to the distance the solvent travelled. This number remains constant across trials. Calculate the Rf value for each pigment. 2. Write a hypothesis (IF/THEN statement) that the Leaf Disk Assay is designed to test. 3. Describe the experimental controls used in the Leaf Disk Assay experiment(s).4. Describe the variable(s) used in the Leaf Disk Assay experiment(s).5. Compare the test groups of the Leaf Disk Assay. Which treatment (syringe) had the most floating leaf disks after 35 minutes?

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1.Pale Yellow 15/15 = 30 mm Rf Value Clear 25/50 = 50 mm Rf Value Green 45/50 = 90 mm Rf Value Light Yellow 50/50 = 100 mm Rf Value 3.Water temperature and reading intervals were the experiment's two controls. 4.Concentration of Baking Soda, Light, and Darkness were the variables. 5.After 35 minutes, there were more floating leaf disks in Syringes C and D with the 0.12% Baking Soda solution.